large-scale unconstrained non-linear optimization algorithm Search Results


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large-scale rna production kits  (Promega)
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(A) Diagram of the in vitro transcription and replication cycle of HCV replicon <t>RNA.</t> The HCV replicon RNAs were transcribed by a T7 RNA polymerase <t>from</t> <t>DNA</t> vectors digested with restriction enzymes as indicated on the right side of panel B. The in vitro-transcribed RNA was transfected into Huh7 cells. Upon replication, the positive-strand HCV RNA is converted to the complementary negative strand, which in turn serves as a template for synthesis of more positive-strand RNA. The 5′- and 3′-end nucleotides of both positive- and negative-strand RNAs are highlighted in boldface letters. (B) Schematic presentation of the in vitro-transcribed HCV replicon RNAs. The nucleotides at the 5′ and 3′ ends of the replicon RNAs are highlighted in boldface letters. The T7 promoter is indicated by an open box. The restriction enzyme used to linearize each cDNA clone of the replicon RNA is shown on the right side. The replicon was named after the 5′- or 3′-end nucleotide, as shown on the left side. The original HCV replicon RNA contains a G at the 5′ end and a U at the 3′ end (37).
Large Scale Rna Production Kits, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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large-scale nonlinear solver ipopt  (MathWorks Inc)
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(A) Diagram of the in vitro transcription and replication cycle of HCV replicon <t>RNA.</t> The HCV replicon RNAs were transcribed by a T7 RNA polymerase <t>from</t> <t>DNA</t> vectors digested with restriction enzymes as indicated on the right side of panel B. The in vitro-transcribed RNA was transfected into Huh7 cells. Upon replication, the positive-strand HCV RNA is converted to the complementary negative strand, which in turn serves as a template for synthesis of more positive-strand RNA. The 5′- and 3′-end nucleotides of both positive- and negative-strand RNAs are highlighted in boldface letters. (B) Schematic presentation of the in vitro-transcribed HCV replicon RNAs. The nucleotides at the 5′ and 3′ ends of the replicon RNAs are highlighted in boldface letters. The T7 promoter is indicated by an open box. The restriction enzyme used to linearize each cDNA clone of the replicon RNA is shown on the right side. The replicon was named after the 5′- or 3′-end nucleotide, as shown on the left side. The original HCV replicon RNA contains a G at the 5′ end and a U at the 3′ end (37).
Large Scale Nonlinear Solver Ipopt, supplied by MathWorks Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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newton-based, large-scale optimization algorithm of  (MathWorks Inc)
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(A) Diagram of the in vitro transcription and replication cycle of HCV replicon <t>RNA.</t> The HCV replicon RNAs were transcribed by a T7 RNA polymerase <t>from</t> <t>DNA</t> vectors digested with restriction enzymes as indicated on the right side of panel B. The in vitro-transcribed RNA was transfected into Huh7 cells. Upon replication, the positive-strand HCV RNA is converted to the complementary negative strand, which in turn serves as a template for synthesis of more positive-strand RNA. The 5′- and 3′-end nucleotides of both positive- and negative-strand RNAs are highlighted in boldface letters. (B) Schematic presentation of the in vitro-transcribed HCV replicon RNAs. The nucleotides at the 5′ and 3′ ends of the replicon RNAs are highlighted in boldface letters. The T7 promoter is indicated by an open box. The restriction enzyme used to linearize each cDNA clone of the replicon RNA is shown on the right side. The replicon was named after the 5′- or 3′-end nucleotide, as shown on the left side. The original HCV replicon RNA contains a G at the 5′ end and a U at the 3′ end (37).
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hindiii linearized sgrna t7-transcription plasmid templates rtw443  (Addgene inc)
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Addgene inc hindiii linearized sgrna t7-transcription plasmid templates rtw443
(A) Diagram of the in vitro transcription and replication cycle of HCV replicon <t>RNA.</t> The HCV replicon RNAs were transcribed by a T7 RNA polymerase <t>from</t> <t>DNA</t> vectors digested with restriction enzymes as indicated on the right side of panel B. The in vitro-transcribed RNA was transfected into Huh7 cells. Upon replication, the positive-strand HCV RNA is converted to the complementary negative strand, which in turn serves as a template for synthesis of more positive-strand RNA. The 5′- and 3′-end nucleotides of both positive- and negative-strand RNAs are highlighted in boldface letters. (B) Schematic presentation of the in vitro-transcribed HCV replicon RNAs. The nucleotides at the 5′ and 3′ ends of the replicon RNAs are highlighted in boldface letters. The T7 promoter is indicated by an open box. The restriction enzyme used to linearize each cDNA clone of the replicon RNA is shown on the right side. The replicon was named after the 5′- or 3′-end nucleotide, as shown on the left side. The original HCV replicon RNA contains a G at the 5′ end and a U at the 3′ end (37).
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(A) Diagram of the in vitro transcription and replication cycle of HCV replicon <t>RNA.</t> The HCV replicon RNAs were transcribed by a T7 RNA polymerase <t>from</t> <t>DNA</t> vectors digested with restriction enzymes as indicated on the right side of panel B. The in vitro-transcribed RNA was transfected into Huh7 cells. Upon replication, the positive-strand HCV RNA is converted to the complementary negative strand, which in turn serves as a template for synthesis of more positive-strand RNA. The 5′- and 3′-end nucleotides of both positive- and negative-strand RNAs are highlighted in boldface letters. (B) Schematic presentation of the in vitro-transcribed HCV replicon RNAs. The nucleotides at the 5′ and 3′ ends of the replicon RNAs are highlighted in boldface letters. The T7 promoter is indicated by an open box. The restriction enzyme used to linearize each cDNA clone of the replicon RNA is shown on the right side. The replicon was named after the 5′- or 3′-end nucleotide, as shown on the left side. The original HCV replicon RNA contains a G at the 5′ end and a U at the 3′ end (37).
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phcvreplicon plasmid  (Promega)
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(A) Diagram of the in vitro transcription and replication cycle of HCV replicon <t>RNA.</t> The HCV replicon RNAs were transcribed by a T7 RNA polymerase <t>from</t> <t>DNA</t> vectors digested with restriction enzymes as indicated on the right side of panel B. The in vitro-transcribed RNA was transfected into Huh7 cells. Upon replication, the positive-strand HCV RNA is converted to the complementary negative strand, which in turn serves as a template for synthesis of more positive-strand RNA. The 5′- and 3′-end nucleotides of both positive- and negative-strand RNAs are highlighted in boldface letters. (B) Schematic presentation of the in vitro-transcribed HCV replicon RNAs. The nucleotides at the 5′ and 3′ ends of the replicon RNAs are highlighted in boldface letters. The T7 promoter is indicated by an open box. The restriction enzyme used to linearize each cDNA clone of the replicon RNA is shown on the right side. The replicon was named after the 5′- or 3′-end nucleotide, as shown on the left side. The original HCV replicon RNA contains a G at the 5′ end and a U at the 3′ end (37).
Phcvreplicon Plasmid, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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large-scale unconstrained non-linear optimization algorithm  (MathWorks Inc)
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(A) Diagram of the in vitro transcription and replication cycle of HCV replicon <t>RNA.</t> The HCV replicon RNAs were transcribed by a T7 RNA polymerase <t>from</t> <t>DNA</t> vectors digested with restriction enzymes as indicated on the right side of panel B. The in vitro-transcribed RNA was transfected into Huh7 cells. Upon replication, the positive-strand HCV RNA is converted to the complementary negative strand, which in turn serves as a template for synthesis of more positive-strand RNA. The 5′- and 3′-end nucleotides of both positive- and negative-strand RNAs are highlighted in boldface letters. (B) Schematic presentation of the in vitro-transcribed HCV replicon RNAs. The nucleotides at the 5′ and 3′ ends of the replicon RNAs are highlighted in boldface letters. The T7 promoter is indicated by an open box. The restriction enzyme used to linearize each cDNA clone of the replicon RNA is shown on the right side. The replicon was named after the 5′- or 3′-end nucleotide, as shown on the left side. The original HCV replicon RNA contains a G at the 5′ end and a U at the 3′ end (37).
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mixed integer nonlinear minlp large scale problem  (MathWorks Inc)
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(A) Diagram of the in vitro transcription and replication cycle of HCV replicon <t>RNA.</t> The HCV replicon RNAs were transcribed by a T7 RNA polymerase <t>from</t> <t>DNA</t> vectors digested with restriction enzymes as indicated on the right side of panel B. The in vitro-transcribed RNA was transfected into Huh7 cells. Upon replication, the positive-strand HCV RNA is converted to the complementary negative strand, which in turn serves as a template for synthesis of more positive-strand RNA. The 5′- and 3′-end nucleotides of both positive- and negative-strand RNAs are highlighted in boldface letters. (B) Schematic presentation of the in vitro-transcribed HCV replicon RNAs. The nucleotides at the 5′ and 3′ ends of the replicon RNAs are highlighted in boldface letters. The T7 promoter is indicated by an open box. The restriction enzyme used to linearize each cDNA clone of the replicon RNA is shown on the right side. The replicon was named after the 5′- or 3′-end nucleotide, as shown on the left side. The original HCV replicon RNA contains a G at the 5′ end and a U at the 3′ end (37).
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Image Search Results


(A) Diagram of the in vitro transcription and replication cycle of HCV replicon RNA. The HCV replicon RNAs were transcribed by a T7 RNA polymerase from DNA vectors digested with restriction enzymes as indicated on the right side of panel B. The in vitro-transcribed RNA was transfected into Huh7 cells. Upon replication, the positive-strand HCV RNA is converted to the complementary negative strand, which in turn serves as a template for synthesis of more positive-strand RNA. The 5′- and 3′-end nucleotides of both positive- and negative-strand RNAs are highlighted in boldface letters. (B) Schematic presentation of the in vitro-transcribed HCV replicon RNAs. The nucleotides at the 5′ and 3′ ends of the replicon RNAs are highlighted in boldface letters. The T7 promoter is indicated by an open box. The restriction enzyme used to linearize each cDNA clone of the replicon RNA is shown on the right side. The replicon was named after the 5′- or 3′-end nucleotide, as shown on the left side. The original HCV replicon RNA contains a G at the 5′ end and a U at the 3′ end (37).

Journal:

Article Title: Effects of Mutations of the Initiation Nucleotides on Hepatitis C Virus RNA Replication in the Cell

doi: 10.1128/JVI.78.7.3633-3643.2004

Figure Lengend Snippet: (A) Diagram of the in vitro transcription and replication cycle of HCV replicon RNA. The HCV replicon RNAs were transcribed by a T7 RNA polymerase from DNA vectors digested with restriction enzymes as indicated on the right side of panel B. The in vitro-transcribed RNA was transfected into Huh7 cells. Upon replication, the positive-strand HCV RNA is converted to the complementary negative strand, which in turn serves as a template for synthesis of more positive-strand RNA. The 5′- and 3′-end nucleotides of both positive- and negative-strand RNAs are highlighted in boldface letters. (B) Schematic presentation of the in vitro-transcribed HCV replicon RNAs. The nucleotides at the 5′ and 3′ ends of the replicon RNAs are highlighted in boldface letters. The T7 promoter is indicated by an open box. The restriction enzyme used to linearize each cDNA clone of the replicon RNA is shown on the right side. The replicon was named after the 5′- or 3′-end nucleotide, as shown on the left side. The original HCV replicon RNA contains a G at the 5′ end and a U at the 3′ end (37).

Article Snippet: Subgenomic HCV replicon RNAs were transcribed in vitro by a T7 RNA polymerase from the above-described DNA constructs linearized by restriction enzyme digestion by using large-scale RNA production kits (Promega) (Fig. ).

Techniques: In Vitro, Transfection

Determination of nucleotides at the 5′ and 3′ ends of HCV replicon RNAs recovered from G418-resistant Huh7 cells. (A) Schematic of the RNA ligase-mediated rapid amplification of cDNA ends (RLM-RACE) (Ambion). An adapter RNA (open bar) was ligated to the 5′ end of either the positive-strand (for determination of the 5′-end nucleotide) or the negative-strand (for determination of the 3′-end nucleotide) HCV replicon RNA isolated from different cell lines. The cDNAs of both positive and negative strands of HCV RNA were reverse transcribed by using HCV-specific primers and amplified by PCR with 5′RACE outer/inner primers (gray arrow) and HCV-specific primers (open arrow). The 5′-end nucleotides of both positive and negative strands of HCV RNA were determined by DNA sequence analysis. (B) 5′- and 3′-end nucleotides of HCV RNA determined by RLM-RACE. Letters indicate nucleotides at the 5′ and 3′ ends as shown on the top. The number indicates the number of cell clones from which HCV replicon RNAs were analyzed. Numbers in parentheses indicate the frequency of the nucleotides determined. The HCV replicon RNAs given on the left column were the ones transcribed in vitro by a T7 RNA polymerase.

Journal:

Article Title: Effects of Mutations of the Initiation Nucleotides on Hepatitis C Virus RNA Replication in the Cell

doi: 10.1128/JVI.78.7.3633-3643.2004

Figure Lengend Snippet: Determination of nucleotides at the 5′ and 3′ ends of HCV replicon RNAs recovered from G418-resistant Huh7 cells. (A) Schematic of the RNA ligase-mediated rapid amplification of cDNA ends (RLM-RACE) (Ambion). An adapter RNA (open bar) was ligated to the 5′ end of either the positive-strand (for determination of the 5′-end nucleotide) or the negative-strand (for determination of the 3′-end nucleotide) HCV replicon RNA isolated from different cell lines. The cDNAs of both positive and negative strands of HCV RNA were reverse transcribed by using HCV-specific primers and amplified by PCR with 5′RACE outer/inner primers (gray arrow) and HCV-specific primers (open arrow). The 5′-end nucleotides of both positive and negative strands of HCV RNA were determined by DNA sequence analysis. (B) 5′- and 3′-end nucleotides of HCV RNA determined by RLM-RACE. Letters indicate nucleotides at the 5′ and 3′ ends as shown on the top. The number indicates the number of cell clones from which HCV replicon RNAs were analyzed. Numbers in parentheses indicate the frequency of the nucleotides determined. The HCV replicon RNAs given on the left column were the ones transcribed in vitro by a T7 RNA polymerase.

Article Snippet: Subgenomic HCV replicon RNAs were transcribed in vitro by a T7 RNA polymerase from the above-described DNA constructs linearized by restriction enzyme digestion by using large-scale RNA production kits (Promega) (Fig. ).

Techniques: Rapid Amplification of cDNA Ends, Isolation, Amplification, Sequencing, Clone Assay, In Vitro